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Prevalence of NDM ‐1 carbapenemase in patients with diarrhoea in Pakistan and evaluation of two chromogenic culture media
Author(s) -
Day K.M.,
Salman M.,
Kazi B.,
Sidjabat H.E.,
Silvey A.,
Lanyon C.V.,
Cummings S.P.,
Ali M.N.,
Raza M.W.,
Paterson D.L.,
Perry J.D.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12171
Subject(s) - newcastle upon tyne , library science , public health , medicine , media studies , sociology , history , nursing , art history , computer science
Aims To evaluate two chromogenic media, Brilliance CRE and chrom ID CARBA , with stool samples referred to the Public Health Laboratories Division of the National Institute of Health in Islamabad, and assess the prevalence of carbapenemase‐producing Enterobacteriaceae ( CPE ) in this population. Methods and Results One hundred and fifty‐two stool samples from patients with diarrhoea were referred to the Microbiology Department and were investigated for the presence of CPE using two chromogenic culture media, Brilliance CRE and chrom ID CARBA . Thirteen patients (8·6%) were found to be colonized with CPE and all produced NDM ‐1 carbapenemase. Twelve of these patients (92%) were found to be colonized by culture on chrom ID CARBA compared with seven (54%) using Brilliance CRE . Conclusions If only coloured colonies were considered as presumptive CPE , the sensitivity, specificity and positive predictive value were 54, 23 and 6% for Brilliance CRE and 85, 85 and 36% for chrom ID CARBA , respectively. Significance and Impact of the Study We conclude that Enterobacteriaceae that produce NDM ‐1 carbapenemase can be found in patients from all major provinces of Pakistan and that chrom ID CARBA was the most effective of the two chromogenic media in this setting.