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Bacteriostatic effect of sequential hydrodynamic and ultrasound‐induced stress
Author(s) -
Chapman J.S.,
Ferguson R.,
Consalo C.,
Bliss T.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12146
Subject(s) - pseudomonas putida , biofilm , population , biology , bacteria , membrane , microbiology and biotechnology , strain (injury) , biophysics , chemistry , biochemistry , genetics , medicine , anatomy , environmental health
Abstract Aims To elucidate the mechanism of action of a nonchemical microbial control technology employing coupled hydrodynamic and ultrasound‐induced stress. Methods & Results The effects of a laboratory model system using a commercial nonchemical device on P seudomonas putida revealed growth and respiration were inhibited without a loss of viability from the treated population. Damage to cell membranes was evident using fluorescent microscopy and a reporter strain containing lux genes fused with a membrane damage stress‐response promoter. Other reporter strains also indicated the possible involvement of DNA and protein repair systems. A consequence of treatment was a reduced ability to form biofilms. Conclusions The nonchemical device caused a biostatic effect on treated cells induced by sublethal damage to several cellular systems, including cell membranes. Significance and Impact of the Study The study demonstrates that biostasis can be an effective mechanism for microbial control in some industrial systems and provides insight into understanding and applying this device and other nonchemical microbial control technologies to real‐world problems of microbial contamination.