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Statistical optimization of culture conditions for biomass production of probiotic gut‐borne S accharomyces cerevisiae strain able to reduce fumonisin B 1
Author(s) -
Armando M.R.,
Galvagno M.A.,
Dogi C.A.,
Cerrutti P.,
Dalcero A.M.,
Cavaglieri L.R.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12144
Subject(s) - probiotic , food science , saccharomyces cerevisiae , yeast extract , biomass (ecology) , yeast , central composite design , response surface methodology , strain (injury) , sugar , biology , incubation , fumonisin b1 , microbiology and biotechnology , chemistry , fermentation , biochemistry , bacteria , mycotoxin , chromatography , agronomy , genetics , anatomy
Aim To evaluate the ability of probiotic S accharomyces cerevisiae RC 016 strain to reduce fumonisin B 1 ( FB 1 ) in vitro and to optimize the culture conditions for the growth of the yeast employing surface response methodology. Methods and Results Using Plackett–Burman screening designs ( PBSD ) and central composite designs ( CCD ), an optimized culture medium containing (g l −1 ) fermentable sugars provided by sugar cane molasses ( CM s), yeast extract ( YE ) and ( NH 4 ) 2 HPO 4 ( DAP ) was formulated. The S. cerevisiae RC 016 strain showed the greatest binding at all assayed FB 1 concentration. The CM s, YE , DAP concentrations and incubation time influenced significantly the biomass of S. cerevisiae RC 016. Conclusion A combination of CM s 17%; YE 4·61 g l −1 and incubation time 60 h was optimum for maximum biomass of S. cerevisiae RC 016. Significance and Impact of the Study The importance of this work lies in the search for live strains with both probiotic and fumonisin B 1 decontamination properties that could be sustainably produced in a medium just containing cheap carbon, nitrogen and phosphorus sources and would be included in a novel product to animal feed.