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Increasing l ‐isoleucine production in Corynebacterium glutamicum by overexpressing global regulator Lrp and two‐component export system Brn FE
Author(s) -
Yin L.,
Shi F.,
Hu X.,
Chen C.,
Wang X.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12141
Subject(s) - corynebacterium glutamicum , isoleucine , operon , biochemistry , chemistry , fermentation , biology , amino acid , escherichia coli , leucine , gene
Aims To increase the l ‐isoleucine production in C orynebacterium glutamicum by overexpressing the global regulator Lrp and the two‐component export system Brn FE . Methods and Results The brnFE operon and the lrp gene were cloned into the shuttle vector pDXW ‐8 individually or in combination. The constructed plasmids were transformed into an l ‐isoleucine‐producing strain C. glutamicum JHI3‐156, and the l ‐isoleucine production in these different strains was analysed and compared. More l ‐isoleucine was produced when only Lrp was expressed than when only BrnFE was expressed. Significant increase in l ‐isoleucine production was observed when Lrp and BrnFE were expressed in combination. Compared to the control strain, l ‐isoleucine production in JHI3‐156/ pDXW ‐8‐ lrp ‐ brnFE increased 63% in flask cultivation, and the specific yield of l ‐isoleucine increased 72% in fed‐batch fermentation. Conclusions Both Lrp and Brn FE are important to enhance the l ‐isoleucine production in C. glutamicum . Significance and Impact of the Study The results provide useful information to enhance l ‐isoleucine or other branched‐chain amino acid production in C. glutamicum .