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Neuraminidase Inhibitors from marine‐derived actinomycete S treptomyces seoulensis
Author(s) -
Jiao R.H.,
Xu H.,
Cui J.T.,
Ge H.M.,
Tan R.X.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12136
Subject(s) - streptomyces , microbiology and biotechnology , biology , neuraminidase , actinomycetales , streptomycetaceae , bacteria , virology , genetics , virus
Aims This work was performed to characterize new secondary metabolites with neuraminidase ( NA ) inhibitory activity from marine actinomycete strains. Methods and Results An actinomycete strain IFB ‐A01, capable of producing new NA inhibitors, was isolated from the gut of shrimp P enasus orientalis and identified as S treptomyces seoulensis according to its 16S rRNA sequence (over 99% homology with that of the standard strain). Repeated chromatography of the methanol extract of the solid‐substrate culture of S . seoulensis IFB‐A01 led to the isolation of streptoseolactone ( 1 ), and limazepines G ( 2 ) and H ( 3 ). The structures of 1 – 3 were determined by a combination of IR , ESI‐MS , 1D ( 1 H and 13 C NMR , and DEPT ) and 2 D NMR experiments ( HMQC , HMBC , 1 H ‐ 1 H COSY and NOESY ). Compounds 1 – 3 showed significant inhibition on NA in a dose‐dependent manner with IC 50 values of 3·92, 7·50 and 7·37 μmol l −1 , respectively. Conclusions This is the first report of two new ( 1 and 2 ) and known ( 3 , recovered as a natural product for the first time in the work) NA inhibitors from the marine‐derived actinomycete S . seoulensis IFB ‐ A 01. Significance and Impact of the Study The three natural NA inhibitors maybe of value for the development of drug(s) necessitated for the treatment of viral infections.