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Radiorespirometric assays for the detection of biogenic sulfides from sulfate‐reducing bacteria
Author(s) -
Queiroz J.C.,
Melo Ferreira A.C.,
Costa A.C.A.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12122
Subject(s) - serial dilution , sulfate reducing bacteria , sulfate , seawater , environmental chemistry , desulfovibrio , bacteria , environmental science , anaerobic bacteria , chemistry , petroleum , sulfide , microorganism , biology , ecology , medicine , genetics , alternative medicine , organic chemistry , pathology
Aims The detection of trace concentrations of biogenic sulfides can be carried out through radiorespirometric assays. The objective of this work was to improve the methodology for detection of H 2 S in trace concentrations, to correlate with sulfate‐reducing bacterial activity. Methods and Results Serial dilutions of synthetic sea water with a pure culture of D esulfovibrio alaskensis , a mixed anaerobic microbial culture and a natural saline sample from a petroleum offshore platform indicated that dilutions were followed, accordingly, by sulfate reduction. Conclusions Tests performed indicated that increasing the time of incubation of a mixed anaerobic microbial culture contributed to an increase in the sulfate reduction rates, as well as the amount of carbon source and inoculum. Significance and Impact of the Study The technique here developed proved to be a rapid test for the detection of biogenic sulfides, particularly those associated with corrosion products, being an useful tool for monitoring and controlling oil/water storage tanks, petroleum continental platforms and several types of reservoirs.