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Absence of tetracycline resistance in C ampylobacter coli isolates from F innish finishing pigs treated with chlortetracycline
Author(s) -
Juntunen P.,
Laurila T.,
Hein M.,
Hänninen M.L.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12120
Subject(s) - tetracycline , campylobacter , biology , microbiology and biotechnology , chlortetracycline , ciprofloxacin , campylobacter coli , erythromycin , typing , minimum inhibitory concentration , antibiotics , bacteria , genetics
Aims To determine whether therapeutic treatment of pigs with chlortetracycline affects the susceptibility of their C ampylobacter isolates for tetracycline, ciprofloxacin and erythromycin. Methods and Results Minimum inhibitory concentrations ( MIC s) and presence of a tetracycline resistance gene tet (O) were studied in C ampylobacter collected before, during and after chlortetracycline treatment. Tetracycline MIC s and the presence of tet ( O ) for additional Campylobacter coli isolates collected previously from seven farrowing farms were also determined. Isolates with ciprofloxacin MIC s above the epidemiological cut‐off value ( ECOFF ) were subtyped by fla A restriction fragment length polymorphism ( RFLP ). Tetracycline MIC s of 221 C amp. coli isolates remained under the ECOFF at all sampling stages as well as the MIC s for 63 isolates from the other farms. The ciprofloxacin MIC was above the ECOFF for 22% of the isolates, and one Camp. coli isolate had an erythromycin MIC above the ECOFF . None of the studied 300 C ampylobacter isolates from nine herds carried tet ( O ). fla A ‐ RFLP typing revealed the heterogeneity of C amp. coli isolates with high ciprofloxacin MIC s. Conclusion Use of chlortetracycline did not increase the MIC values for the antimicrobials studied. Significance and Impact of the Study This study demonstrated that susceptibility of C amp. coli isolates is not affected by chlortetracycline therapy if tet ( O ) is not present in C amp. coli population.