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Utilization and optimization of a waste stream cellulose culture medium for pigment production by P enicillium spp.
Author(s) -
Sopandi T.,
Wardah A.,
Surtiningsih T.,
Suwandi A.,
Smith J.J.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12110
Subject(s) - xylose , penicillium , corncob , food science , biomass (ecology) , cellulose , yeast extract , chemistry , incubation , fermentation , botany , biology , agronomy , raw material , biochemistry , organic chemistry
Aims This research sought to determine optimal corn waste stream–based fermentation medium C and N sources and incubation time to maximize pigment production by an indigenous Indonesian P enicillium spp., as well as to assess pigment pH stability. Methods and Results A P enicillium spp. was isolated from Indonesian soil, identified as P enicillium resticulosum , and used to test the effects of carbon and nitrogen type and concentrations, medium pH, incubation period and furfural on biomass and pigment yield ( PY ) in a waste corncob hydrolysate basal medium. Maximum red PY (497·03 ± 55·13 mg l −1 ) was obtained with a 21 : 1 C : N ratio, pH 5·5–6·0; yeast extract‐, NH 4 NO 3 ‐, N a NO 3 ‐, M g SO 4 ·7 H 2 O ‐, xylose‐ or carboxymethylcellulose ( CMC )‐supplemented medium and 12 days (25°C, 60–70% relative humidity, dark) incubation. C source, C , N and furfural concentration, medium pH and incubation period all influenced biomass and PY . Pigment was pH 2–9 stable. Conclusions P enicillium resticulosum demonstrated microbial pH‐stable‐pigment production potential using a xylose or CMC and N source, supplemented waste stream cellulose culture medium. Significance and Impact of the Study Corn derived, waste stream cellulose can be used as a culture medium for fungal pigment production. Such application provides a process for agricultural waste stream resource reuse for production of compounds in increasing demand.