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Silver colloidal nanoparticles: effect on matrix composition and structure of C andida albicans and C andida glabrata biofilms
Author(s) -
Monteiro D.R.,
Silva S.,
Negri M.,
Gorup L.F.,
Camargo E.R.,
Oliveira R.,
Barbosa D.B.,
Henriques M.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12102
Subject(s) - candida glabrata , candida albicans , biofilm , microbiology and biotechnology , silver nanoparticle , chemistry , nanoparticle , biology , bacteria , materials science , nanotechnology , genetics
Aim The aim of this study was to assess the effect of different silver nanoparticles ( SN ) concentrations on the matrix composition and structure of C andida albicans and C andida glabrata biofilms. Methods and Results C andida biofilms were developed in 6‐well microtiter plates during 48 h. After, these biofilms were exposed to 13·5 or 54 μg SN ml −1 for 24 h. Then, extracellular matrices were extracted from biofilms and analysed chemically in terms of proteins, carbohydrates and DNA . To investigate the biofilm structure, scanning electron microscopy ( SEM ) and epifluorescence microscopy were used. SN interfered with the matrix composition of C andida biofilms tested in terms of protein, carbohydrate and DNA , except for the protein content of C . albicans biofilm. By SEM , C andida biofilms treated with SN revealed structural differences, when compared with the control groups. Further, SN showed a trend of agglomeration within the biofilms. Epifluorescence microscopy images suggest that SN induced damage on cell walls of the C andida isolates tested. Conclusions In general, irrespective of concentration, SN affected the matrix composition and structure of C andida biofilms and these findings may be related to the mechanisms of biocide action of SN . Significance and Impact of the Study This study reveals new insights about the behaviour of SN when in contact with C andida biofilms. SN may contribute to the development of therapies to prevent or control C andida infections.