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Thermostable Bacteriocin BL 8 from B acillus licheniformis isolated from marine sediment
Author(s) -
Smitha S.,
Bhat S.G.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12097
Subject(s) - bacillus licheniformis , bacteriocin , bacteria , bacillus (shape) , microbiology and biotechnology , biology , molecular mass , antimicrobial , food science , biochemistry , bacillus subtilis , enzyme , genetics
Aim To isolate and characterize bacteriocin, BL 8, from the bacteria identified as B acillus licheniformis from marine environment. Methods and Results One‐hundred and twelve bacterial isolates from sediment and water samples collected off the coast of Cochin, India, were screened for antibacterial activity. Strain BTHT8, identified as Bacillus licheniformis , inhibited the growth of Gram‐positive test organisms. The active component labelled as bacteriocin BL8 was partially purified by ammonium sulphate fractionation and was subjected to glycine SDS‐PAGE. The band exhibiting antimicrobial activity was electroeluted and analysed using MALDI‐TOF mass spectrometry, and the molecular mass was determined as 1·4 kDa. N‐terminal amino acid sequencing of BL8 gave a 13 amino acid sequence stretch. Bacteriocin BL8 was stable even after boiling at 100°C for 30 min and over a wide pH range of 1–12. Conclusion A novel, pH ‐tolerant and thermostable bacteriocin BL8, active against the tested Gram‐positive bacteria, was isolated from B acillus licheniformis . Significance and Impact of the Study This study reports a stable, low molecular weight bacteriocin from B acillus licheniformis . This bacteriocin can be used to address two important applications: as a therapeutic agent and as a biopreservative in food processing industry.