Premium
The corn influence on the adsorption levels of aflatoxin B 1 and zearalenone by yeast cell wall
Author(s) -
Pereyra C.M.,
Cavaglieri L.R.,
Chiacchiera S.M.,
Dalcero A.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12082
Subject(s) - zearalenone , adsorption , aflatoxin , yeast , toxin , chemistry , chromatography , mycotoxin , matrix (chemical analysis) , cell wall , polysaccharide , zea mays , food science , biochemistry , biology , organic chemistry , agronomy
Aims To in vitro evaluate the influence of the corn on the adsorption levels of aflatoxin B1 ( AFB 1) and zearalenone ( ZEA ) by yeast cell walls ( YCW s). Methods and Results Two commercial YCW s were studied. The YCW s contain different percentages of polysaccharides. YCW 1 and 2 contain 5·9 and 21% of mannans and 17·4 and 23% of β‐glucans, respectively. Each YCW was resuspended in p H 2 and p H 6 buffer solutions. Corn was used to study the matrix influence. An aliquot of 500 μl YCW suspension was added to each microtube containing 500 μl of 0·1, 0·25, 0·5, 1, 2·5 and 5 μg ml −1 AFB 1 or 0·5, 5, 10, 20 and 50 μg ml −1 ZEA . Microtubes were kept with mechanical agitation at 37°C for 30 min and then centrifuged for 10 min at 16 873 g and; the supernatants were quantified by high‐pressure liquid chromatography. The amount of bound toxin was plotted as a function of the amount of added toxin according to mathematical expressions proposed by three theoretical models. Both YCW s were capable of adsorbing AFB 1 and ZEA in amounts from 0·061 to 0·40 and from 0·10 and 0·26 g g −1 , respectively. In the presence of the matrix, both adsorbents were not able to adsorb AFB 1 . However, they could adsorb ZEA at levels from 0·03 to 0·23 g g −1 . Conclusions Both YCW s adsorbed ZEA in the presence of corn and also under simulated gastrointestinal pH conditions. These results suggest that the studied YCW s are potential candidates for ZEA adsorption. Significance and Impact of the Study Several in vitro assays have informed the ability of different substrates including yeast walls to adsorb AFB 1 and ZEA ; none of them have evaluated their ability to adsorb AFB 1 and ZEA in the presence of the corn. The corn matrix can influence the adsorption phenomena of these mycotoxins.