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Growth, acid production and bacteriocin production by probiotic candidates under simulated colonic conditions
Author(s) -
Fernandez B.,
Le Lay C.,
Jean J.,
Fliss I.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12081
Subject(s) - bacteriocin , pediococcus acidilactici , lactococcus lactis , microbiology and biotechnology , nisin , biology , probiotic , food science , bacteria , lactococcus , lactic acid , antimicrobial , lactobacillus plantarum , genetics
Aims The aim of this study is to evaluate the capacity of three bacteriocin producers, namely L actococcus lactis subsp. lactis biovar diacetylactis UL 719 (nisin Z producer), L . lactis ATCC 11454 (nisin A producer) and P ediococcus acidilactici UL 5 (pediocin PA ‐1 producer), and to grow and produce their active bacteriocins in M acfarlane broth, which mimics the nutrient composition encountered in the human large intestine. Methods and Results The three bacteriocin‐producing strains were grown in M acfarlane broth and in D e M an– R ogosa– S harpe ( MRS ) broth. For each strain, the bacterial count, p H drop and production of organic acids and bacteriocins were measured for different period of time. The ability of the probiotic candidates to inhibit L isteria ivanovii HPB 28 in co‐culture in M acfarlane broth was also examined. Lactococcus lactis subsp. lactis biovar diacetylactis UL719, L . lactis ATCC 11454 and P ed. acidilactici UL 5 were able to grow and produce their bacteriocins in MRS broth and in M acfarlane broth. Each of the three candidates inhibited L . ivanovii HPB 28, and this inhibition activity was correlated with bacteriocin production. The role of bacteriocin production in the inhibition of L . ivanovii in Macfarlane broth was confirmed for P ed. acidilactici UL 5 using a pediocin nonproducer mutant. Conclusions The data provide some evidence that these bacteria can produce bacteriocins in a complex medium with carbon source similar to those found in the colon. Significance and Impact of the Study This study demonstrates the capacity of lactic acid bacteria to produce their bacteriocins in a medium simulating the nutrient composition of the large intestine.