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Comparative study of the protective capacity against S almonella infection between probiotic and nonprobiotic lactobacilli
Author(s) -
Castillo N.A.,
Moreno de LeBlanc A.,
M. Galdeano C.,
Perdigón G.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12074
Subject(s) - lactobacillus casei , probiotic , microbiology and biotechnology , salmonella enterica , biology , salmonella , lactobacillus acidophilus , salmonella infection , lactobacillus , pathogen , proinflammatory cytokine , immunology , inflammation , bacteria , genetics
Aims To investigate the immunoprotective ability of three L actobacilli strains against S almonella enterica serovar T yphimurium in a mouse model. To identify the probiotic properties involved in the protection against infection caused by this pathogen. Methods and Results The immunomodulatory effect of three different lactobacilli strains: L actobacillus ( L act .) casei CRL 431 (probiotic bacterium), L act. delbrueckii subsp. bulgaricus CRL 423 ( L act. bulgaricus ) and L act.acidophilus CRL 730 was compared using a mouse model of S almonella infection. L actobacillus casei continuous administration improved animal survival, diminished pathogen spreading outside the intestine, attenuated the intestinal inflammation, modulated cytokine profile previous and postinfection and increased the expression and secretion of I g A in the gut. Additionally, the administration of this lactobacilli increased peritoneal, P eyer's patches and spleen macrophages' phagocytic activity in healthy mice and monocyte chemotactic protein ( MCP ‐1) released by intestinal epithelial cells in an in vitro assay. Although L act. acidophilus increased the number of I g A ‐secreting cells previous and postinfection, and L act . bulgaricus increased MCP ‐1 released by intestinal epithelial cells and the phagocytic activity of macrophages, these effects alone were not enough to confer protection against S almonella T yphimurium infection in mouse. Conclusions Probiotic strain L act. casei CRL 431 was the one that induced protection against S almonella , by increasing the intestinal barrier function and by decreasing the local inflammatory response. Significance and Impact of the Study Salmonella spp. constitutes an important agent of foodborne diseases in the world. Not all lactobacilli, even with some immunostimulating properties at gut level, can protect against S almonella infection. Lactobacillus casei CRL 431, a probiotic bacterium, could be useful as an oral mucosal adjuvant of the immune system to improve gut health, especially in the prevention or amelioration of S almonella infections. We demonstrated that there is not a unique mechanism by which this protective effect was exerted.