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A serological method for detection of N osema ceranae
Author(s) -
Aronstein K.A.,
Webster T.C.,
Saldivar E.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12066
Subject(s) - nosema ceranae , serology , biology , virology , microbiology and biotechnology , antibody , nosema , immunology , microsporidia , spore
Aims We developed a new method for detection of the intracellular parasite, N osema ceranae , one of the most economically devastating pathogens of the honeybee. Methods and Results The SWP ‐32 antibody was used for the development of an enzyme‐linked immunosorbent assay ( ELISA ). We also compared the efficiency of this ELISA to microscopy and quantitative real‐time (q RT ) PCR , the methods currently in use. Conclusions ELISA is comparable in sensitivity with the q RT ‐ PCR , less expensive and faster. When this method is commercialized and made available to bee‐keepers, it will allow them to make informed decisions for the application of in‐hive chemicals. Hence, bee‐keepers may be able to determine when treatments for control of N . ceranae are unnecessary and reduce the cost, time and possible side effects of these treatments. Significance and Impact of the Study This assay provides the first serological method for detection of N . ceranae in bee colonies, which is as sensitive as DNA amplification. It can be easily adopted for both laboratory and field applications.