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Screening and characterization of LAB ‐produced bacteriocin‐like substances from the intestine of grey mullet ( M ugil cephalus L .) as potential biocontrol agents in aquaculture
Author(s) -
Lin Y.H.,
Chen Y.S.,
Wu H.C.,
Pan S.F.,
Yu B.,
Chiang C.M.,
Chiu C.M.,
Yanagida F.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12041
Subject(s) - enterococcus faecium , bacteriocin , microbiology and biotechnology , biology , enterococcus , lactococcus , bacteria , antimicrobial , lactic acid , lactococcus lactis , antibiotics , genetics
Abstract Aim To isolate and characterize bacteriocin‐like inhibitory substance ( BLIS )‐producing lactic acid bacteria from the intestine of grey mullet. Methods and Results Inhibitory activity against at least one or more indicator strains was observed in one E nterococcus thailandicus , one E nterococcus faecium and two L actococcus garvieae strains. E nterococcus faecium B 3‐8 and E nt. thailandicus B 3‐22 showed the greatest inhibitory activities against L isteria monocytogenes ATCC 19111 and were therefore further characterized. The results suggested that the inhibitory substances from the two strains showed similar characteristics with respect to sensitivity to heat and proteolytic enzymes. BLIS from E nt. thailandicus B 3‐22 was characterized by a broader inhibitory spectrum than that from E nt. faecium B 3‐8. SDS ‐ PAGE revealed that the molecular size of partially purified BLIS s from E nt. faecium B 3‐8 and E nt. thailandicus B 3‐22 was c . 5 and 3 kDa, respectively. The molecular mass of purified bacteriocin from E nt. thailandicus B 3‐22 was further determined to be 6319 Da by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry ( MALDI ‐ TOF MS ). The results indicated that BLIS from E nt. thailandicus B 3‐22 can effectively inhibit the growth of all tested L . garvieae strains. Conclusions The findings obtained in this study suggest the potential use of E nt. thailandicus B 3‐22 as a biocontrol agent against pathogenic L . garvieae in the aquaculture. Significance and Impact of the Study This is the first report describing the characteristics of BLIS from E nt. thailandicus that showed potential for use as a biocontrol agent in the aquaculture.