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Hydrocarbon‐degrading potential of microbial communities from A rctic plants
Author(s) -
FerreraRodríguez O.,
Greer C.W.,
Juck D.,
Consaul L.L.,
MartínezRomero E.,
Whyte L.G.
Publication year - 2013
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/jam.12020
Subject(s) - library science , sociology , computer science
Abstract Aims To explore rhizospheric microbial communities from A rctic native plant species evaluating their bacterial hydrocarbon‐degrading capacities. Methods and Results E riophorum scheuchzeri , P otentilla cf. rubricaulis, O xyria digyna, S alix arctica and P uccinellia angustata plant species were collected at E ureka ( C anadian high A rctic) along with their rhizospheric soil samples. Their bacterial community fingerprints (16 S rRNA gene, DGGE ) were distinctive encompassing members from the phyla: B acteroidetes, F irmicutes, A ctinobacteria and P roteobacteria . Isolated diesel‐degrading bacteria belonged to the phyla A ctinobacteria and P roteobacteria . Strains of M ycobacterium , N ocardia , R hodococcus, I ntrasporangiaceae, L eifsoni and A rthrobacter possessed alkB and P seudomonas possessed both ndoB and xylE gene sequences. Two R hodococcus strains mineralized [1‐ 14 C] hexadecane at 5 and −5°C. From the rhizosphere of P . angustata , larger numbers of hydrocarbon‐degrading bacteria were isolated than from other plant rhizosphere samples and all three genes alkB (from A ctinobacteria ), ndoB and xylE (from P seudomonas ) were detected by PCR . Conclusions (i) Arctic plants have unique rhizospheric bacterial communities. (ii) P . angustata has potential for phytoremediation research at high A rctic soils. (iii) Isolated bacteria mineralized hydrocarbons at ambient low temperatures. Significance and Impact of the Study To the best of our knowledge, this is the first rhizospheric exploration examining the phytoremediation potential of five A rctic plants and evaluating their microbial hydrocarbon‐degrading capacities.