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Isolation and characterization of microsatellite loci in Pimelodus microstoma (Siluriformes: Pimelodidae)
Author(s) -
Satie Savada Camila,
Cristina Costa de Lima Moema,
Luís Orsi Mário,
Simões de Almeida Fernanda
Publication year - 2020
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.14104
Subject(s) - biology , microsatellite , locus (genetics) , loss of heterozygosity , genetics , allele , zoology , gene
Abstract Ten microsatellite loci were developed and described for Pimelodus microstoma, identified in eggs collected from the lower Das Cinzas river. Nine loci were polymorphic, with 02–19 alleles per locus. The observed heterozygosity values were up to 0.898. Five species of the Pimelodidae family were also tested for amplification of loci, among which Pimelodus maculatus was the most successful in the transferability of primers. These loci have proven to be useful for studying the variability and structuring of populations of Pimelodus microstoma .