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Triploidization in the streaked prochilod Prochilodus lineatus inferred by flow cytometry, blood smears and karyological approaches
Author(s) -
Yasui George Shigueki,
Nakaghi Laura Satiko Okada,
Monzani Paulo Sérgio,
Nascimento Nivaldo Ferreira do,
Pereira dos Santos Matheus,
Goes Caio Augusto Gomes,
PortoForesti Fábio,
Senhorini José Augusto
Publication year - 2020
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.14025
Subject(s) - biology , ploidy , hatching , giemsa stain , karyotype , human fertilization , chromosome , embryo , zoology , fish <actinopterygii> , fluorescence in situ hybridization , microbiology and biotechnology , andrology , genetics , fishery , gene , medicine
The streaked prochilod Prochilodus lineatus is an important fish species from the Neotropical region. In this study, a protocol of triploidization was established by temperature shock. Fertilized eggs were shocked at 2 min post‐fertilization at 0, 38, 40 and 42°C. At 0°C, the embryos were maintained for 30 min, while the rest for 2 min. Heat shocked embryos and control (during all experiment) were incubated at 27°C. The ploidy status was confirmed by flow cytometry, erythrocyte diameter, conventional cytogenetics (Giemsa staining), chromosome banding and fluorescence in situ hybridization using 5S and 18S rDNA probes. Heat‐shock at 40°C produced 96.7% of triploids and such a procedure did not reduce the fertilization, hatching rate and the percentage of normal embryos. The use of chromosome banding and FISH gave rise to effective procedure to identify triploids. The data obtained here is innovative for the species and bring new information for basic and applied studies.