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Isolation and characterization of microsatellite loci in Hexagrammos otakii based on 2b‐RAD method
Author(s) -
Shen Zhen,
Wang Bo,
Hu Fawen,
Liu Hongzhan,
Guan Hongbin,
Zheng Fengrong
Publication year - 2020
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.13988
Subject(s) - microsatellite , biology , loss of heterozygosity , allele , genetics , genetic diversity , polymorphism (computer science) , population , gene , demography , sociology
In this study, we isolated 20 novel microsatellites loci associated with the growth of Hexagrammos otakii (Fat greenling) by using 2b‐RAD sequencing method. The characteristics of 20 microsatellite loci were amplified in 105 H. otakii individuals which came from four different groups and tested by Capillary electrophoresis. The number of allele of the 20 microsatellite loci ranged from 8 to 26 with an average of 19.95. PIC value ranged from 0.1659 to 0.9227 with an average of 0.7555. Observed and expected heterozygosity varied from 0.0667 to 0.8571 and from 0.1710 to 0.9314, respectively. Highly polymorphic characteristics were observed in each microsatellite loci of the study except HO6. Further research showed that 10 microsatellite loci had transferability in Hexagrammos agrammus . In this study, high polymorphism and genetic diversity using the 15 polymorphic microsatellite loci suggest that they are suitable for investigating the fine‐scale population structure, genetic relationship and further evaluating the artificial reproduction strategy of H. otakii .