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Tissue expression and bioinformatics analysis of the vitellogenin gene of Asian arowana ( Scleropages formosus )
Author(s) -
Pan Xianhui,
Liu Yi,
Zhou Kangqi,
Mu Xidong,
Zheng Shuming,
Liu Chao,
Hu Yinchang
Publication year - 2019
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.13927
Subject(s) - biology , vitellogenin , open reading frame , gene , complementary dna , untranslated region , genetics , rapid amplification of cdna ends , phylogenetic tree , microbiology and biotechnology , messenger rna , peptide sequence
The RACE technique was used to clone the full‐length vitellogenin (VTG) cDNA sequence of Asian arowana ( Scleropages formosus ). The full‐length sequence was 5,550 bp with an open reading frame of 5,238 bp, encoding 1,745 amino acids, and 5′ and 3′ UTRs (untranslated regions) of 45 bp and 267 bp, respectively. Phylogenetic analysis showed that S. formosus and silver arowana ( Osteoglossum bicirrhosum ) share a close evolutionary relationship (bootstrap 100%). The quantitative real‐time PCR results showed that vtg expression was significantly higher in liver and gonads of male and female fish compared with its expression in the other tissues tested ( p < 0.01). The relative expression levels of vtg in liver, gland, kidney, heart, head kidney, and brain of female fish were significantly higher than in the corresponding tissues of male fish ( p < 0.05).