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Ten novel microsatellite markers for the freshwater sleeper Micropercops swinhonis (Günther, 1873), with testing of cross‐species amplification in two other fishes from suborder Gobioidei
Author(s) -
Schmidt Bjorn,
Wang Zhen,
Cheng Fei,
Xie Songguang
Publication year - 2018
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.13750
Subject(s) - biology , microsatellite , loss of heterozygosity , allele , population , genetics , genetic marker , range (aeronautics) , zoology , evolutionary biology , gene , materials science , sociology , composite material , demography
Abstract Ten novel polymorphic microsatellite loci were developed for the freshwater sleeper Micropercops swinhonis , a relatively abundant and broadly distributed lake‐dwelling fish in Asia, using next generation sequencing. These markers were tested in a population of 48 individuals, and characteristics suggest that they may be useful for population genetic studies. The average number of alleles per marker was 6.6 (range: 2–26), the average expected heterozygosity was 0.55 (range: 0.081–0.955), and the average polymorphic information content value per marker was 0.507 (range: 0.077–0.942). No marker showed significant deviation from Hardy‐Weinberg Equilibrium following Bonferroni correction of significance values, and no markers showed evidence of null alleles, large allele dropout, or scoring errors from stutter bands. Two markers had successful amplification in Odontobutis potamophila , and one marker had successful amplification in Rhinogobius giurinus . These novel markers may be useful for further research in population genetic studies involving M. swinhonis .