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Cloning and differential expression of the growth hormone in Pampus argenteus
Author(s) -
Xiaojing Zhu,
Qiaoqiong Tong,
Jiazhe Yang,
Jiabao Hu,
Xiaoge Guo,
Yajun Wang,
Shanliang Xu,
Danli Wang
Publication year - 2018
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.13720
Subject(s) - biology , in situ hybridization , complementary dna , medicine , endocrinology , cloning (programming) , gene expression , gene , genetics , computer science , programming language
Abstract The growth hormone (GH) is a pluripotent hormone produced by the pituitary in vertebrates. It plays important roles in the growth, development, and metabolism of vertebrates.We cloned GH cDNA sequence of Pampus argenteus (GenBank: KT257176). Multi‐sequence analysis revealed P. argenteus GH cDNA contained four conservative cysteine residues positions (Cys 69 , Cys 177 , Cys 194 , and Cys 202 ) and shared more than 51.5% identity with homologues from other reported bony fish GHs, except that of Lepisosteus osseus . We used semi‐quantitative RT‐PCR and quantitative real‐time PCR to detect GH expression in 10 tissues and GH expression levels in the pituitary at six different growth stages, and also detected GH content in serum at different growth stages . qPCR showed that GH mRNA was detected in the liver, muscle, kidney, intestine, pituitary, olfactory bulb, stomach, heart, gill, and ovary. The highest level of P. argenteus GH mRNA was observed in the pituitary ( P < 0.01, n = 3). At different growth stages, P. argenteus GH expression first increased, decreased, and increased again. GH gene expression levels and the variations of serum GH levels of P. argenteus were consistent with the growth rate and associated with the sexual maturity. In addition, in situ hybridization was used to locate the GH expression in pituitary. In situ hybridization showed that the GH‐positive cells were round, oval, or irregular and often gathered into groups or presented branches along the nerve fibers.