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Viable diploid progeny induced from sperm of Chinese tetraploid pond loach by cold‐shock androgenesis
Author(s) -
Zhou H.,
Gao Y.C.,
Zhuang Z.X.,
Zhang R.,
Liang Y.T.,
Xu Q.Z.,
Wang Y.S.,
Qi H.R.,
Arai K.,
Li Y.J.
Publication year - 2018
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.13514
Subject(s) - biology , misgurnus , ploidy , sperm , human fertilization , pronucleus , genetics , zygote , cobitidae , parthenogenesis , zoology , embryo , polyploid , artificial fertilization , chromosome , andrology , botany , embryogenesis , gene , fish <actinopterygii> , medicine , fishery
Summary Natural tetraploid loach ( Misgurnus anguillicaudatus ) living in the Changjiang River basin and adjacent area in China has been considered a true genetic tetraploidy (4n = 100) with four sets of homologous chromosomes. Here, we reported its further supporting evidence provided by the cold‐shock androgenesis. Viable androgenetic progeny appeared when eggs were cold‐shocked at 3°C for 60 min, starting 5 min after fertilization with sperm of natural tetraploid males, although the survival rate was significantly lower than control group. The majority (87%) of androgenetic embryos were diploid (2n = 50) and all‐paternal inheritance was verified in larval stage by microsatellite genotypes. Microscopic observations confirmed the elimination of both egg nucleus and second polar body from a fertilized egg, followed by the cleavage exclusively with sperm nucleus. Thus, the appearance of normal diploid androgenetic progeny from sperm of natural tetraploid loach revealed the presence of four chromosome sets in tetraploid males.