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Anaesthesia and transport of juvenile tambaqui Colossoma macropomum (Cuvier, 1818) with tricaine methane‐sulphonate: Implications on secondary and oxidative stress responses
Author(s) -
Barbas L. A. L.,
PereiraCardona P. M.,
Maltez L. C.,
Garcia L. O.,
Monserrat J. M.,
Sampaio L. A.
Publication year - 2017
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.13382
Subject(s) - tambaqui , lipid peroxidation , oxidative stress , pco2 , biology , gill , anesthesia , juvenile fish , zoology , chemistry , juvenile , biochemistry , fish <actinopterygii> , medicine , fishery , ecology
Summary The aim of this study was to evaluate the anaesthetic efficacy and the effect of sedation with tricaine ( TMS ® ), also known as MS ‐222, on secondary and oxidative stress parameters in juvenile tambaqui, Colossoma macropomum transported in hyperoxia for 2, 6 and 10 hr. Juveniles were placed in aquaria containing six different concentrations of buffered tricaine (150, 180, 210, 240, 270 and 300 mg/L) and the times for anaesthesia induction and recovery determined. Fish transported in hyperoxic conditions were investigated for glycemia, ionic concentration (K + , Ca ++ , Na + ) and osmolality, haematocrit (Ht) and haemoglobin concentration (Hb), partial pressure of gases ( p CO 2 and p O 2 ), pH and bicarbonate concentration ( HCO 3 − ) in whole blood collected from caudal vasculature. Total antioxidant capacity against peroxyl radicals ( ACAP ), glutathione‐S‐transferase ( GST ) activity and lipid peroxidation levels ( TBARS ) were investigated in gills, brain and liver. All concentrations of TMS ® induced deep anaesthesia in juvenile tambaqui in this study. A concentration of 240 mg/L of TMS ® was sufficient to induce rapid anaesthesia (<3 min) with uneventful recovery (<5 min). In light of the secondary and oxidative stress responses of fish transported using TMS ® , which were generally not significantly different compared to responses of fish transported in anaesthetic‐free water, sedation with 20 mg/L is not advantageous and therefore is dispensable for the transport of this species for up to 10 hr.