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Description of 21 microsatellites for the Chinese bitterling, Rhodeus sinensis Günther, 1868
Author(s) -
Xiong L.W.,
Wang Q.,
Wang S.B.,
Wang J.G.,
Feng Q.,
Yue L.J.
Publication year - 2017
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.13380
Subject(s) - biology , zoology , evolutionary biology , genetics
Summary Chinese bitterling ( Rhodeus sinensis ) is a freshwater fish endemic to China. During the past several decades, natural populations of this species have become endangered due to habitat fragmentation and water pollution. To help conserve and restore R. sinensis , Illumina Miseq sequencing was used to identify 21 polymorphic microsatellites. The number of alleles varied from 3 to 13, and the observed heterozygosity and expected heterozygosity ranged from 0.3067 to 0.9145 and 0.3845 to 0.9521, respectively. The polymorphism information content ranged from 0.3173 to 0.9105. These novel microsatellites will be useful in both conservation and management of R. sinensis .