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Effects of dietary amino acid l ‐lysine on survival, growth and haemato‐biochemical parameters in Indian catfish, Heteropneustes fossilis (Bloch, 1974), fingerlings
Author(s) -
Ahmed I.
Publication year - 2017
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.13355
Subject(s) - heteropneustes fossilis , catfish , biology , zoology , lysine , feed conversion ratio , weight gain , protein efficiency ratio , body weight , dietary protein , fish <actinopterygii> , biochemistry , amino acid , endocrinology , fishery
Summary An 8‐week feeding experiment was conducted to estimate the dietary lysine requirement of fingerling Indian catfish, Heteropneustes fossilis (total length = 6.35 ± 1.10 cm; weight = 4.70 ± 0.65 g) by feeding six isonitrogenous (400 g/kg CP ) and isocaloric diets (17.90 kJ/g) containing six supplemental lysine levels ranging from 11.0 to 23.5 g/kg (diets I to VI , in incremental steps of 2.5 g/kg). Triplicate groups with 20 fish each were stocked in 75‐L circular continuous flow‐through troughs and fed experimental diets at 4% BW /day twice daily (08:00 and 18:00 h). Maximum live weight gain (304%), best‐feed conversion ratio ( FCR , 1.46) and protein efficiency ratio ( PER , 1.71) were obtained at 18.5 g/kg dietary lysine, beyond which these values showed a slight declining tendency. Best values for somatic and hematological indices were also obtained at this level. Significantly ( p < .05) higher body protein and low carcass moisture and intermediate fat contents were noted in fish fed diets containing 18.5 g/kg lysine. The quadratic regression analyses of live weight gain, FCR , PER and body protein deposition (BPD) data indicated lysine requirements at 19.3, 18.8, 18.6 and 18.6 g/kg of dry diet, respectively. Based on these results, it is recommended that the H. fossilis diet should contain lysine at a level of 19.0 g/kg of dry diet, corresponding to 47.5 g/kg of dietary protein, for optimum growth and efficient feed utilization.