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Nineteen novel microsatellites in Chinese lake gudgeon Sarcocheilichthys sinensis Bleeker, 1971
Author(s) -
Shen Y.B.,
Wang R.Q.,
Ren J.Q.,
Fu H.T.,
Yang X.M.,
Li J.
Publication year - 2017
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.13229
Subject(s) - loss of heterozygosity , microsatellite , biology , china , zoology , genetics , gene , allele , archaeology , history
Summary Nineteen polymorphic microsatellites isolated from a partial genomic DNA library of Sarcocheilichthys sinensis enriched for CA repeats were developed. Variability was tested on 48 unrelated individuals collected from Jiangsu province, China. Observed heterozygosity ranged from 0.200 ( Ss236 ) to 1.00 ( Ss223 , Ss224 ), whereas the expected heterozygosity ranged from 0.213 ( Ss232 ) to 0.939 ( Ss224 ). Shannon's information index ranged from 0.405 ( Ss236 ) to 2.988 ( Ss224 ). Sixteen of the 19 microsatellites conformed to Hardy–Weinberg equilibrium. These microsatellites can be used for the evaluation of genetic parameters in populations to supply valuable information for conservation management.