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Characterization and SNP variation of the interleukin‐1 β gene of bighead carp ( Cyprinus pellegrini Tchang, 1933) and five strains of common carp [ Cyprinus carpio Linnaeus (1758)] in China
Author(s) -
Jia Z.Y.,
Ge H.Z.,
Bai Y.Y.,
Li C.T.,
Shi L.Y.
Publication year - 2015
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.12862
Subject(s) - biology , single nucleotide polymorphism , genetics , common carp , cyprinus , bighead carp , minor allele frequency , genotype , carp , exon , allele frequency , allele , genotype frequency , snp , genetic variation , intron , gene , fishery , fish <actinopterygii>
Summary Single nucleotide polymorphisms of Interleukin‐1 β ( IL ‐1 β ) have been reported as markers for susceptibility to infectious diseases in humans and livestock. The present study was to determine the genetic variation of this cytokine in six carp strains. Among the sampled individuals, a total of 13 SNP s, including eight in introns and five in coding regions, were identified at intron 5, exon 6, intron 6 and exon 7. Three positions of 1700, 1733 and 1934 resulted in variable amino acid changes with Phe to Tyr, Pro to Leu and Lys to Asn, respectively. Five positions with minor allele frequency ( MAF ) were larger than 0.05. Among 13 SNP s, eight positions of allele frequency and ten positions of genotypic frequency showed significant differences between some populations. The genotype distributions of the 13 SNP s were consistent with the assumption of the Hardy‐Weinberg equilibrium, with the exception of two positions in the Yibu and bighead carp (P < 0.05), so as to supply the genetic information for research on infectious diseases.