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A high‐throughput strategy to controlled thawing of cryopreserved fish semen for large‐scale laboratory fertilization trials – a simulation approach
Author(s) -
Kunz F. A.,
Lahnsteiner J.,
Mansour N.,
Schuster D. S.,
Zinterhof jn P.,
Lahnsteiner F.
Publication year - 2015
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.12742
Subject(s) - cryopreservation , throughput , biology , semen , block (permutation group theory) , human fertilization , fish <actinopterygii> , marine engineering , materials science , biomedical engineering , mathematics , computer science , anatomy , engineering , fishery , geometry , embryo , telecommunications , wireless
Summary Cryopreservation of fish semen is a modern technology. In the present study a simulation with SolidWorks FlowSimulation 2014 was conducted to determine if automated, simultaneous thawing of a large number of straws is possible. It was simulated with straws that were taken out of the liquid nitrogen container and clamped into semicircular indentations between two metal blocks (=thawing block). Cooling coils, allowing an exact temperature control, crossed the blocks. Different thawing conditions were simulated and compared with thawing rates obtained in a water bath. Comparison of thawing rates was by calculating the area under curve ( AUC ) (mathematically known as integral), as this computation is very useful for comparing changes over a time interval. AUC s obtained by waterbath thawing at 25°C differed by only 1.84% from AUC s obtained by thawing simulations in the thawing block. These data represent the first promising results that automated thawing of multiple straws can be handled.