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Identification of 5–15 kDa substances in carp seminal plasma using mass spectrometry
Author(s) -
Westfalewicz B.,
Dietrich M. A.,
Irnazarow I.,
Ciereszko A.
Publication year - 2015
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.12738
Subject(s) - mass spectrometry , carp , biology , molecular mass , chromatography , time of flight mass spectrometry , microbiology and biotechnology , biochemistry , chemistry , ionization , fish <actinopterygii> , fishery , ion , organic chemistry , enzyme
Summary The objective of this study was to detect substances of 5–15 kDa in carp seminal plasma using matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry ( MALDI ‐ TOF MS ) and to compare mass spectrometry profiles between two common carp lines. Resolved were seminal plasma proteins using tris – tricine SDS – PAGE . Also found were 19 mass spectra peaks of carp seminal plasma. A few low‐molecular mass proteins were identified, including parvalbumin, apolipoprotein, histone H 4 and H 2 A , and one hypothetical protein. Mass spectrometry was not useful for molecular profiling of carp breeding lines, but are potentially useful for comparative studies of cyprinids.