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The effect of a magnetic field on trout ( Salmo trutta Linnaeus, 1758) sperm motility parameters and fertilisation rate
Author(s) -
Formicki K.,
Szulc J.,
KorzeleckaOrkisz A.,
Tański A.,
Kurzydłowski J. K.,
Grzonka J.,
Kwiatkowski P.
Publication year - 2015
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.12737
Subject(s) - biology , sperm , trout , fertilisation , sperm motility , spermatozoon , dna fragmentation , salmo , milt , motility , andrology , hatching , zoology , fishery , cryopreservation , embryo , botany , genetics , reproductive technology , fish <actinopterygii> , medicine , apoptosis , programmed cell death
Summary The objective of the study was to investigate the effects of a static magnetic field on the trout sperm motility parameters ( CASA ), DNA integrity (comet assay) and morphology ( SEM ). The fertilisation rate was also assessed. Exposing the trout spermatozoa to a static magnetic field (1 mT, 5 mT, 10 mT) prolonged the sperm motility to 12 days (288 h), while the control sperm remained motile for only 3 days (72 h). Analysis of fragmentation of genetic material in the spermatozoon head revealed no effects from the studied magnetic fields. After a 30‐min exposure of spermatozoa to the magnetic field, the proportion of fertilised eggs was already 12.7% (1 mT) to 15% (10 mT), higher than that in the control variant. After a 24‐h storage in the magnetic field the difference was even larger – <1% of eggs were fertilised in the control; in the magnetic field the proportion of fertilised eggs increased to 11.4 at 3 mT and 22.1 at 1 mT. The results demonstrate that short‐term storage of sperm in a static magnetic field of low intensity has a positive effect on the sperm motility parameters (not causing DNA fragmentation), which in turn improves the effectiveness of fertilisation and hatching. This suggests that this method, after more comprehensive studies, might be used for short‐term storage of trout sperm.