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Effect of diet supplementation with a commercial probiotic containing Pediococcus acidilactici (Lindner, 1887) on the expression of five quality markers in zebrafish ( Danio rerio (Hamilton, 1822)) testis
Author(s) -
Valcarce D. G.,
Pardo M. Á.,
Riesco M. F.,
Cruz Z.,
Robles V.
Publication year - 2015
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.12731
Subject(s) - pediococcus acidilactici , biology , probiotic , zebrafish , endocrinology , lactic acid , biochemistry , bacteria , genetics , lactobacillus plantarum , gene
Summary The effects of a commercial probiotic diet supplement (Bactocell ® ), containing lactic acid bacteria Pediococcus acidilactici were evaluated by identifying five zebrafish ( Danio rerio ) sperm quality markers. P. acidilactici (10 6 colony‐forming units per gram) was supplied to young zebrafish males (age 8 months) as a feed probiotic additive during a 10 days test period. Five transcripts, previously described as male zebrafish quality markers, were studied: brain‐derived neurotrophic factor ( bdnf ), BCL 2‐interacting killer ( bik ), double‐sex and mab‐3 related transcription factor 1 ( dmrt1 ), follicle‐stimulating hormone beta subunit ( fshb ) and leptin a ( lepa ). After exposition, males fed on with the supplemented diet with a commercial probiotic containing P. acidilactici presented an over‐expression on three fertility markers comparing to the control group: lepa , dmrt1 and bdnf . These results suggest that P. acidilactici has a potential use as probiotic supplement in zebrafish diet for the improvement of molecular parameters in testicular cells, indicating that probiotic supplementation could affect male reproductive performance.