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Spermatozoa motility traits influenced by activation media in the European whitefish ( Coregonus lavaretus L. 1758)
Author(s) -
Dziewulska K.,
PileckaRapacz M.,
Domagała J.
Publication year - 2015
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.12722
Subject(s) - hatchery , motility , biology , coregonus lavaretus , artificial fertilization , human fertilization , sperm motility , zoology , anatomy , fishery , microbiology and biotechnology , fish <actinopterygii>
Summary The use of a properly selected activating solution during artificial fertilization permits improvement in spermatozoa motility characteristics and enhancement of the fertilization success. This is particularly important for species characterised by low motility parameters. The aim of the study was to determine the types of environments favourable for whitefish ( Coregonus lavaretus L.) spermatozoa activation on the basis of analysis of the spermatozoa motility parameters. Motility parameters were evaluated for four activation media: 30 m m NaCl, Billard's solution, 120 m m Na HCO 3 and hatchery water, using computer assisted sperm analysis ( CASA ). The study was divided into two experiments: (i) in the first, the effects of three solutions were tested: 30 m m NaCl, Billard's solution and 120 m m Na HCO 3 ; (ii) in the second, the effects of hatchery water and 30 m m NaCl were compared. The durations of the active motility phase in the three tested solutions were similar (approximately 150 s), while that measured in hatchery water was significantly shorter (115 s). Upon activation, motility ( MOT ) was similar in all activating media but the highest values of curvilinear velocity ( VCL ), straight line velocity ( VSL ) and amplitude of lateral head displacement ( ALH ) were observed in 30 m m NaCl. NaCl solution was a suitable environment for the whitefish spermatozoa activation. The velocity parameters obtained in this medium exceed those noted in other tested environments, particularly upon activation.