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Expression pattern of somatolactin in the C hinese sturgeon, A cipenser sinensis G ray, 1835
Author(s) -
Leng X.Q.,
Li C.J.,
Cao H.
Publication year - 2014
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.12587
Subject(s) - sturgeon , biology , pars intermedia , zoology , antiserum , western blot , acipenser , peptide sequence , microbiology and biotechnology , pituitary gland , genetics , gene , antibody , fish <actinopterygii> , endocrinology , fishery , hormone
Summary Somatolactin ( SL ) is a pituitary hormone specific to fish and is reported in a variety of teleost species. Although it appears to be involved in many different physiological processes, its biological functions are poorly understood in A cipenseriformes. In this paper the expression pattern of this protein is characterized in C hinese sturgeon ( A cipenser sinensis ). Structure analysis showed that A s SL had a 24aa signal peptide and a 208aa mature protein, one N ‐glycosylation site at positions 145‐147 and seven C ys residues conserved with other SL s. The antiserum was raised against a 15 aa antigen, derived from the C hinese sturgeon somatolactin ( A s SL ) amino acid sequence. Western blot analysis showed the pituitary‐specific expression of A s SL . In situ hybridization revealed that the mRNA of A s SL was expressed mainly in pars intermedia ( PI ), although also slightly in proximal pars distalis ( PPD ), cells expressing A s SL disperse throughout the pars intermedia. These findings provide useful information for future physiological research in C hinese sturgeon.