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Effects of human chorionic gonadotropin (h CG ) and handling stress on spermiation of silver perch L eiopotherapon plumbeus ( K ner, 1864)
Author(s) -
Denusta P. J. T.,
JesusAyson E. G. T.,
Laron M. A.,
Garcia L. M. B.
Publication year - 2014
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.12383
Subject(s) - milt , sperm , biology , saline , human chorionic gonadotropin , perch , endocrinology , andrology , zoology , medicine , fish <actinopterygii> , hormone , fishery , botany
Summary This study determined the effect of human chorionic gonadotropin (h CG ) and handling stress on the spermiation and milt response of silver perch L eiopotherapon plumbeus based on the measurement of spermatocrit, sperm density, and milt production. Compared to saline‐injected fish, the mean spermatocrit (or packed sperm) of hCG ‐treated fish was significantly lower at 18 h (47.9%) and 30 h (40.2%) post‐injection while mean sperm density was significantly lower at 30 h post‐injection (3.6 × 10 6 cells μ l −1 ) but not at 18 h. At 18 h (1.8 μ l g‐BW −1 ) and 30 h (2.5 μ l g‐BW −1 ) post‐injection, mean milt production of h CG ‐treated fish was significantly higher than in the saline group. Milt consistency was also thinner in the h CG ‐treated group. Mean sperm density of handled fish (18.0 × 10 6 cells μ l −1 ) was significantly lower than control fish (23.4 × 10 6 cells μ l −1 ). However, mean sperm density of handled plus saline‐injected (16.2 × 10 6 cells μ l −1 ) and handled plus h CG ‐treated fish (8.4 × 10 6 cells μ l −1 ) was significantly lower than in the control goup. Having thicker milt consistency, mean spermatocrit and milt production of handled (77.5%; 1.1 μ l g‐BW −1 , respectively) and handled plus saline‐injected fish (75.4%; 1.1 μ l g‐BW −1 , respectively) were not significantly different from the control fish (76.2%; 1.3 μ l g‐BW −1 , respectively). Handled plus h CG ‐treated fish had the lowest mean sperm density (8.4 × 10 6 cells μ l −1 ) and spermatocrit (54.7%), but had the highest mean milt production (5.5 μ l g‐BW −1 ) among the treatment groups. These results demonstrate that the h CG injection effectively induces spermiation and milt expression and that handling‐related stress negatively affects such responses. The spermatocrit method may be used to assess the spermiation and milt response of silver perch.