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Cryopreservation of giant grouper E pinephelus lanceolatus ( B loch, 1790) sperm
Author(s) -
Fan B.,
Liu X.C.,
Meng Z.N.,
Tan B. H.,
Wang L.,
Zhang H.F.,
Zhang Y.,
Wang Y.X.,
Lin H.R.
Publication year - 2014
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.12321
Subject(s) - biology , cryoprotectant , sperm , cryopreservation , human fertilization , epinephelus , grouper , andrology , dimethyl sulfoxide , sperm motility , glycerol , botany , anatomy , fishery , fish <actinopterygii> , biochemistry , embryo , chemistry , medicine , organic chemistry
Summary The grouper, E pinephelus lanceolatus , is a vulnerable species of high economic value. An effective protocol was developed for the cryopreservation of E . lanceolatus by comparing different extenders produced by mixing various cryoprotectants (dimethyl sulfoxide, methanol and glycerol) and diluents ( MPRS , TS ‐2, TS ‐19, C ortland and H ank's). Using computer‐assisted sperm analysis ( CASA ) and morphological analysis, the sperm motility and fertilization rates from post‐thaw sperm were comparable to untreated controls. The results revealed that MPRS (containing 12% DMSO ) or TS ‐19 (containing 12% DMSO ), were the optimum extenders for protecting the sperm from cryo‐damage in liquid nitrogen. The post‐thaw sperm maintained high motility (90.61 ± 3.03%) and a fertilization rate (92.27 ± 2.43%) similar (P > 0.05) to fresh sperm (94.34 ± 4% and 94.10 ± 1.87%). This study is the first to report on the successful sperm cryopreservation of E . lanceolatus and provides an important tool for repopulating this species through aquaculture.