Cryopreservation of giant grouper   E pinephelus lanceolatus  ( B loch, 1790) sperm | Zendy

Fan B. | Zendy; Liu X.C. | Zendy; Meng Z.N. | Zendy; Tan B. H. | Zendy; Wang L. | Zendy; Zhang H.F. | Zendy; Zhang Y. | Zendy; Wang Y.X. | Zendy; Lin H.R. | Zendy

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Cryopreservation of giant grouper E pinephelus lanceolatus ( B loch, 1790) sperm

Author(s) -

Fan B.,

Liu X.C.,

Meng Z.N.,

Tan B. H.,

Wang L.,

Zhang H.F.,

Zhang Y.,

Wang Y.X.,

Lin H.R.

Publication year - 2014

Publication title -

journal of applied ichthyology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.392

H-Index - 62

eISSN - 1439-0426

pISSN - 0175-8659

DOI - 10.1111/jai.12321

Subject(s) - biology , cryoprotectant , sperm , cryopreservation , human fertilization , epinephelus , grouper , andrology , dimethyl sulfoxide , sperm motility , glycerol , botany , anatomy , fishery , fish <actinopterygii> , biochemistry , embryo , chemistry , medicine , organic chemistry

Summary The grouper, E pinephelus lanceolatus , is a vulnerable species of high economic value. An effective protocol was developed for the cryopreservation of E . lanceolatus by comparing different extenders produced by mixing various cryoprotectants (dimethyl sulfoxide, methanol and glycerol) and diluents ( MPRS , TS ‐2, TS ‐19, C ortland and H ank's). Using computer‐assisted sperm analysis ( CASA ) and morphological analysis, the sperm motility and fertilization rates from post‐thaw sperm were comparable to untreated controls. The results revealed that MPRS (containing 12% DMSO ) or TS ‐19 (containing 12% DMSO ), were the optimum extenders for protecting the sperm from cryo‐damage in liquid nitrogen. The post‐thaw sperm maintained high motility (90.61 ± 3.03%) and a fertilization rate (92.27 ± 2.43%) similar (P > 0.05) to fresh sperm (94.34 ± 4% and 94.10 ± 1.87%). This study is the first to report on the successful sperm cryopreservation of E . lanceolatus and provides an important tool for repopulating this species through aquaculture.

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