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Discrimination between invasive Ponto‐Caspian gobies using a PCR ‐ RFLP method
Author(s) -
Kohout J.,
Pekárik L.,
Šedivá A.,
Didenko A.,
Čiampor F.,
ČiamporováZaťovičová Z.
Publication year - 2014
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.12315
Subject(s) - biology , neogobius , round goby , goby , zoology , restriction fragment length polymorphism , invasive species , fishery , ecology , polymerase chain reaction , fish <actinopterygii> , genetics , gene
Summary Accurate identification of invaders, and especially their juveniles and eggs, is a difficult task if several morphologically similar species co‐occur. The aim of the study was to develop and test a rapid and cost‐effective procedure for identification of five species of invasive gobies occurring in the middle Danube basin, namely round goby Neogobius melanostomus , bighead goby Ponticola kessleri , monkey goby Neogobius fluviatilis , racer goby Babka gymnotrachelus and tubenose goby Proterorhinus semilunaris . First, a 708 bp fragment of the cytochrome oxidase I gene was amplified and sequenced for representative samples of these five species. Appropriate sequences of the five species available in public databases were used for in silico analysis. A digestion of the amplified fragment with the Bfa I enzyme was found to be suitable for the species identification, as it showed unique restriction patterns for each species. The technique was also successfully applied for fish remains from burbot Lota lota stomachs. Thus the technique could be a useful tool in monitoring biological invasions, especially by identifying specimens that could not be determined on the basis of morphological features. The results demonstrate that the PCR ‐ RFLP method may in some cases be more reliable for species identification than a standard DNA sequencing.