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Shrinkage of P rochilodus lineatus ( V alenciennes, 1847) larvae preserved in either ethyl‐alcohol or formalin in relation to their developmental stage and feeding condition
Author(s) -
Gómez M. I.,
Sánchez S.,
Fuentes C. M.
Publication year - 2014
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.12308
Subject(s) - biology , preservative , larva , shrinkage , hatching , alcohol , zoology , fishery , ecology , food science , biochemistry , machine learning , computer science
Summary The effects of preservation in 95% ethyl‐alcohol and 5% formalin were analysed for 3 months on standard length of P rochilodus lineatus larvae from hatching to the end of the flexion process. Unyolked stages were raised under two feeding regimes: unfed and daily fed. All developmental stages that were preserved in formalin as well as the yolked and flexion‐postflexion larvae stored in alcohol shrank significantly (2–6%). In contrast, unyolked preflexion larvae showed a slight but significant enlargement after storage in alcohol (1%). Shrinkage of preflexion stages was 2.5% higher when stored in formalin, while both preservative agents caused similar shrinkage in flexion‐postflexion larvae (ca. 3%). Shrinkage levels after storage in alcohol were dependent on live length, decreasing or increasing with increasing length in yolked and flexion‐postflexion larvae, respectively. The feeding regime did not affect length changes after preservation in either preservative agent.