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Effects of norfloxacin on the drug metabolism enzymes of two sturgeon species ( A cipenser schrencki and A cipenser ruthenus )
Author(s) -
Li S. W.,
Wang D.,
Liu H. B.,
Lu T. Y.
Publication year - 2013
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.12239
Subject(s) - acipenser , biology , sturgeon , glutathione , norfloxacin , zoology , metabolism , enzyme , biochemistry , fish <actinopterygii> , fishery , antibiotics , ciprofloxacin
Summary The enzyme activities of 7‐ethoxycoumarin O ‐deethylase ( ECOD ) and glutathione S ‐transferase ( GST ), and on glutathione ( GSH ) content, involved in metabolism of the antibiotic N orfloxacin ( NFLX ), were investigated in A cipenser schrencki and A cipenser ruthenus . Sturgeons weighing 45–55 g were kept in an aquarium (0.5 m × 0.5 m × 0.9 m) for two weeks under controlled conditions (fish density 88 individuals per m 3 , 18°C) before the experiment. The two species of sturgeon were divided into five groups each (n = 15 in each group), with each group subdivided into three replicates of five fish per tank. A control group in which distilled water was administered orally was also tested. NFLX was forced into the stomachs of the fish at a concentration of 20, 40, 60, 80 and 100 mg kg −1 body weight, respectively. ECOD activities in liver microsomes, and GST activity and GSH content in liver microsomes and blood plasma, were measured and compared. Results indicate that ECOD activity is progressively inhibited with increasing NFLX concentrations. ECOD activity varied from 0.12 nmol mg −1 min −1 to 0.07 nmol mg −1 min −1 , demonstrating an inhibition rate of 60.83% in A . schrencki and 65.14% in A . ruthenus . In both species tested, GST and GSH levels exhibited a trend of first increasing, and then decreasing with increasing NFLX levels, reaching a peak value at 40 mg per kg −1 body weight. Thus, the presented results indicate that NFLX can induce a change in the activity of some drug metabolism related enzymes such as ECOD and GST in vivo .