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Effect of short‐term (0–72 h) fasting on serum biochemical characteristics in rainbow trout O ncorhynchus mykiss
Author(s) -
Hoseini S. M.,
Yousefi M.,
Rajabiesterabadi H.,
Paktinat M.
Publication year - 2014
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.12221
Subject(s) - rainbow trout , medicine , endocrinology , triglyceride , albumin , globulin , biology , triiodothyronine , cholesterol , serum albumin , fish <actinopterygii> , hormone , fishery
Summary An experiment was carried out to investigate the effects of short‐term fasting periods on the serum biochemical characteristics of rainbow trout, O ncorhynchus mykiss . For this purpose, fish were fasted 0, 6, 12, 24, 48 and 72 h before blood sampling. Thereafter the serum levels of thyroxine (T 4 ), 3,5,3′‐triiodothyronine (T 3 ), cortisol, glucose, lactate, triglyceride, cholesterol, total protein, albumin, globulin and albumin : globulin ration (A : G) were determined. Results show that serum levels of T 4 (4.60–8.77 ng ml −1 ), T 3 (7.50–13.3 ng ml −1 ), cortisol (7.91–24.5 ng ml −1 ), glucose (18.5–80.1 mg dl −1 ), lactate (12.7–29.6 mg dl −1 ), triglyceride (171–500 mg dl −1 ), and cholesterol (321–535 mg dl −1 ) were significantly affected by the fasting period. However, there were no significant changes in serum total protein (3.03–3.68 g dl −1 ), albumin (1.78–2.01 g dl −1 ), globulin (1.15–1.70 g dl −1 ) or A : G (1.13–1.93) among the fish fasted 0–72 h. Results clearly show the importance of a fasting period on the serum biochemical properties of rainbow trout. According to the results, 24 h fasting is suggested as a pre‐sampling fasting period in rainbow trout to measure serum levels of T 4 , T 3 , cortisol, glucose, lactate, triglyceride, and cholesterol. Potential mechanisms related to the changes in biochemical properties are discussed.