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Sperm motility parameters for Steindachneridion parahybae based on open‐source software
Author(s) -
Sanches E. A.,
Marcos R. M.,
Okawara R. Y.,
Caneppele D.,
Bombardelli R. A.,
Romagosa E.
Publication year - 2013
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.12165
Subject(s) - biology , sperm , sperm motility , motility , open source software , plug in , semen analysis , open source , semen collection , software , anatomy , computer science , microbiology and biotechnology , genetics , operating system , artificial insemination , infertility , pregnancy
Summary The objective of this work was to evaluate the sperm motility of 13 S teindachneridion parahybae males using open‐source software ( I mage J / CASA plugin). The sperm activation procedure and image capture were initiated after semen collection. Four experimental phases were defined from the videos captured of each male as follows: (i) standardization of a dialogue box generated by the CASA plugin within I mage J ; (ii) frame numbers used to perform the analysis; (iii) post‐activation motility between 10 and 20 s with analysis at each 1 s; and (iv) post‐activation motility between 10 and 50 s with analysis at each 10 s. The settings used in the CASA dialogue box were satisfactory, and the results were consistent. These analyses should be performed using 50 frames immediately after sperm activation because spermatozoa quickly lose their vigor. At 10 s post‐activation, 89.1% motile sperm was observed with 107.2 μm s −1 curvilinear velocity, 83.6 μm s −1 average path velocity, 77.1 μm s −1 straight line velocity; 91.6% were of straightness and 77.1% of wobble. The CASA plugin within I mage J can be applied in sperm analysis of the study species by using the established settings.