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Motility, morphology, mitochondria and ATP content of diploid spermatozoa from sex‐reversed clonal diploid and neo‐tetraploid loach, M isgurnus anguillicaudatus
Author(s) -
Zhao Y.,
Psenicka M.,
Fujimoto T.,
Saito T.,
Yasui G. S.,
Yamaha E.,
Arai K.
Publication year - 2012
Publication title -
journal of applied ichthyology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.392
H-Index - 62
eISSN - 1439-0426
pISSN - 0175-8659
DOI - 10.1111/jai.12069
Subject(s) - ploidy , misgurnus , biology , spermatozoon , sperm , polyploid , reproduction , genetics , polar body , motility , andrology , meiosis , fish <actinopterygii> , gene , medicine , fishery
Summary Diploid spermatozoa were obtained from sex‐reversed clonal diploid males and neo‐tetraploid which derived from normal diploid × natural tetraploid cross followed by the 2nd polar body release inhibition. The spermatozoa from sex‐reversed clonal diploid males exhibited a significant reduction in motility when compared with those from normal diploid and neo‐tetraploid males. Electron microscopy revealed that diploid spermatozoa from sex‐reversed clonal diploid and neo‐tetraploid males exhibited normal shape similar to haploid spermatozoa from normal diploid males, except for larger sperm‐head size and longer flagellum. Considering flow‐cytometric results, volume of mitochondrial mass per spermatozoon was also increased in proportion to the elevation of ploidy status from haploid to diploid sperm. Diploid spermatozoa from neo‐tetraploid increased the number of mitochondria and the ATP content, but those from sex‐reversed clonal diploid males did not show such results.