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Hollow mesoporous silica capsules loaded with copper, silver, and zinc oxide nanoclusters for sustained antibacterial efficacy
Author(s) -
Krakor Eva,
Saniternik Sven,
Gessner Isabel,
Frohnhoven Robert,
Wilhelm Michael,
Drexelius Marco,
Tosun Nurgül,
Neundorf Ines,
Mathur Sanjay
Publication year - 2022
Publication title -
journal of the american ceramic society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 196
eISSN - 1551-2916
pISSN - 0002-7820
DOI - 10.1111/jace.18002
Subject(s) - zinc , mesoporous material , copper , metal , materials science , nucleation , chemical engineering , nanoparticle , nanoclusters , x ray photoelectron spectroscopy , nanomaterials , oxide , nanotechnology , nuclear chemistry , chemistry , metallurgy , catalysis , organic chemistry , engineering
Abstract Intensive and overuse of antibiotics during the last years has triggered a distinct rise in antibiotic resistance worldwide. In addition to the newly developed antimicrobials, there is a high demand for alternative treatment options against persistent bacterial infections. The biocidal impact of metal ions like copper (Cu 2+ ), silver (Ag + ), and zinc (Zn 2+ ), also known as the oligodynamic effect has been used for ages to kill or inhibit the growth of microorganisms and to employ long‐term prevention strategies against their biological antagonists. Herein, we report on the synthesis of Cu, Ag, and Zn metal and corresponding oxide nanoparticles immobilized on hollow mesoporous silica capsules (HMSCs) obtained by a hard‐template assisted sol‐gel synthesis followed by reduction of appropriate metal salts in the presence of HMSCs. Compartmentalization of nanosized metal and oxide clusters in Ag@HMSCs, Cu@HMSCs, and ZnO@HMSCs particles prevented their agglomeration and offered high release kinetics of metal ions between 2.0 and 3.7 mM during 24 h, as monitored by UV‐vis analyses. The distribution and morphology of pristine and metal functionalized HMSCs were evaluated by transmission electron microscopy analysis revealing the successful synthesis of Ag, Cu, and ZnO nanoparticles supported on HMSCs. X‐ray photoelectron spectroscopy revealed that mainly Cu(II), Ag(0), and Zn(II) species were present in the modified HMSCs. In addition to the surface attachment of preformed metal (Ag and Cu) and metal oxide (ZnO) cluster, nucleation of metal nanoparticles inside the void of HMSCs provided an internal reservoir which allowed for a time‐dependent release of metal ions through slower dissolution rates leading to a long‐term and sustained bacterial inhibition over several hours. The high antimicrobial efficiency of Ag@HMSCs, Cu@HMSCs, and ZnO@HMSCs particles was investigated toward both Gram‐positive ( Bacillus subtilis ) and Gram‐negative ( Escherichia coli ) bacteria by INT assays showing a complete growth inhibition for both bacteria types after 24 h. While Ag@HMSCs and Cu@HMSCs showed a higher susceptibility against Gram‐negative bacteria, ZnO@HMSCs showed a higher susceptibility against Gram‐positive bacteria. This demonstrates the promise of metal‐loaded capsules as antibacterial delivery vehicles with dual‐mode time‐release profiles being potential alternatives for antibiotic drugs.