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Potential involvement of rapeseed ( Brassica napus L.) metallothioneins in the hydrogen peroxide‐induced regulation of seed vigour
Author(s) -
MierekAdamska Agnieszka,
Kotowicz Karolina,
Goc Anna,
Boniecka Justyna,
Berdychowska Julia,
Dąbrowska Grażyna B.
Publication year - 2019
Publication title -
journal of agronomy and crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.095
H-Index - 74
eISSN - 1439-037X
pISSN - 0931-2250
DOI - 10.1111/jac.12361
Subject(s) - rapeseed , germination , hydrogen peroxide , seedling , brassica , mutant , reactive oxygen species , biology , dormancy , yeast , horticulture , botany , chemistry , food science , biochemistry , gene
Germination is a crucial process in crop plant life cycle that determines future yield. Hydrogen peroxide plays a significant role in both dormancy release and seed ageing. The potential of the rapeseed metallothioneins ( Bn MT 1‐4) as reactive oxygen species scavengers was investigated. In the presence of H 2 O 2 , bacterial cells expressing Bn MT 1‐4 had higher growth rate than control cells. However, such effect was not observed in an oxidative stress oversensitive yap‐1Δ yeast mutant strain expressing Bn MT 1‐4. Next, it was shown that H 2 O 2 , up to 100 and 10 mM , promotes rapeseed germination rate and seedling growth, respectively. Moreover, it was demonstrated that in seeds germinating in the presence of H 2 O 2, the expression of seed‐specific Bn MT 4 decreases to a high extent in a dose‐ and time‐dependent manner. It was shown that the decline in Bn MT 4 mRNA level, accelerated by up to 10 mM H 2 O 2 treatment, correlates positively with increased rapeseed germination and early growth, underscoring the plausible role of Bn MT 4 as a H 2 O 2 scavenging protein that level has to be downregulated in conditions where the compound is needed. Finally, it was demonstrated that H 2 O 2 could be used as a priming agent of rapeseeds.