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Effect of Chemical and Physical Stress Conditions on the Concentration and Composition of Essential Oil Components in Leaves of Full‐Grown A rtemisia annua L.
Author(s) -
Ivarsen E.,
Kjær A.,
Jensen M.,
Grevsen K.,
Christensen L. P.,
Fretté X.
Publication year - 2013
Publication title -
journal of agronomy and crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.095
H-Index - 74
eISSN - 1439-037X
pISSN - 0931-2250
DOI - 10.1111/jac.12030
Subject(s) - camphene , chemical composition , camphor , composition (language) , chemistry , essential oil , artemisia annua , horticulture , gas chromatography–mass spectrometry , food science , botany , biology , chromatography , mass spectrometry , organic chemistry , malaria , immunology , linguistics , philosophy , artemisinin , plasmodium falciparum
Full‐grown Artemisia annua plants were subjected to chemical and physical stress conditions, and the effect of these on the concentration and chemical composition of essential oil components ( EOC ) in the leaves was studied. The chemical stress treatments were performed by foliar application of NaCl, H 2 O 2 , salicylic acid and chitosan oligosaccharide ( COS ). The EOC of the leaves were extracted with n ‐hexane and identified and quantified by GC – MS and GC – FID , respectively. Approximately 96 % of EOC in the extracts were identified and quantified of which β‐pinene, camphene, germacrene D, camphor, coumarin and dihydro‐ epi ‐deoxyarteannuin B were the major EOC accounting for about 75 % of the total content of EOC in the extracts. The physical stress treatment, sandblasting of the plants resulted in a significant enhancement in the content of α‐pinene, camphene, coumarin and dihydro‐ epi ‐deoxyarteannuin B. The total yield of identified EOC in non‐treated plants (control) was 86.2 ± 13.8 μg g −1 fresh weight ( FW ) compared with 104.0 ± 9.1 μg g −1 FW in sandblasted plants. The chemical stress treatments did not affect the composition of EOC significantly. The results indicate that chemical stress treatments do not affect the concentration and composition of EOC in full‐grown A. annua plants to the same extent as physical stress treatment by sandblasting.