Premium
Effects of Exogenous Abscisic Acid on Antioxidant System in Weedy and Cultivated Rice with Different Chilling Sensitivity under Chilling Stress
Author(s) -
Wang G.J.,
Miao W.,
Wang J.Y.,
Ma D.R.,
Li J.Q.,
Chen W.F.
Publication year - 2013
Publication title -
journal of agronomy and crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.095
H-Index - 74
eISSN - 1439-037X
pISSN - 0931-2250
DOI - 10.1111/jac.12004
Subject(s) - apx , abscisic acid , glutathione reductase , catalase , antioxidant , superoxide dismutase , malondialdehyde , glutathione , peroxidase , chemistry , oxidative stress , horticulture , botany , biology , glutathione peroxidase , biochemistry , enzyme , gene
Two chilling‐tolerant genotypes, that is, weedy rice WR 03‐45 and cultivated rice L ijiangxintuanheigu and two chilling‐sensitive genotypes, that is, weedy rice WR 03‐26 and cultivated rice Xiuzinuo were used in this study to investigate the effects of exogenous abscisic acid ( ABA ) on protection against chilling damage as well as on changes in physiological features. The results showed that under chilling stress the increased levels of superoxide radical (O 2· −), hydrogen peroxide ( H 2 O 2 ) and malondialdehyde ( MDA ) in WR 03‐45 and L ijiangxintuanheigu were lower than those in WR 03‐26 and X iuzinuo. Activities of antioxidant enzymes (superoxide dismutase ( SOD ), catalase ( CAT ), ascorbate peroxidase ( APX ), glutathione reductase ( GR )) and non‐enzymatic antioxidants (ascorbate acid ( A s A ) and reduced glutathione ( GSH )) were enhanced in WR 03‐45 and L ijiangxintuanheigu, whereas they were decreased significantly in WR 03‐26 and X iuzinuo. Application of exogenous ABA reduced the chilling damage in the four genotypes. The pre‐treatment with ABA decreased the levels ofO 2· −, H 2 O 2 and MDA caused by chilling stress in the four genotypes through increasing the activities of SOD , CAT , APX , GR and the contents of A s A and GSH in the four genotypes under chilling stress. Moreover, pre‐treatment with F luridone, the ABA biosynthesis inhibitor, prohibited the effects of ABA through enhancing the oxidative damages and suppressing the antioxidant defence systems under chilling stress. The results indicate the mechanism for rice with chilling tolerance is to enhance the capacity of antioxidant defence systems under chilling stress. Furthermore, ABA plays important roles in the tolerance of rice against chilling stress for it could induce the capacity of whole antioxidant defence systems including enzymatic and non‐enzymatic constitutions under chilling stress.