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Isolation of Antibody to Human Placental Alkaline Phosphatase (PLAP) from Extracts of Human Placentae
Author(s) -
Freeman Jennifer L.,
Rhyne Paul W.,
Dean Deyrick O.,
Beard G. Alan,
Doellgast George J.
Publication year - 2001
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.8755-8920.2001.460206.x
Subject(s) - placental alkaline phosphatase , syncytiotrophoblast , antibody , placenta , antigen , biology , monoclonal antibody , alkaline phosphatase , enzyme , microbiology and biotechnology , biochemistry , pregnancy , fetus , immunology , genetics
PROBLEM: Human placental alkaline phosphatase (PLAP) is a unique placental antigen bound to the syncytiotrophoblast, which may be able to elicit a specific immune response in pregnancy.
METHOD OF STUDY: Antibody to PLAP was purified from placental extracts by: a) acid elution of membrane vesicles; b) purifying complexes of PLAP with human antibody on monoclonal antibodies to PLAP followed by denaturation of the enzyme; and c) by denaturation of PLAP in placental extracts and purification of antibody to PLAP on PLAP columns.
RESULTS AND CONCLUSIONS: Specific antibody to PLAP is present in placental extracts, and is mostly bound to placental membrane preparations. PLAP is therefore immunogenic in pregnancy and could serve as a useful monitor of pregnancy‐specific immunological responses. Since a similar enzyme appears in some cancers, it is possible that the immunization against PLAP in pregnancy will help to protect against the development of ovarian and endometrial cancer (the ‘fetal antigen’ hypothesis).