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Localization and Distribution of Platelet Activating Factor Receptors in the Mouse Ovary and Oviduct During the Estrous Cycle and Early Pregnancy
Author(s) -
LASH GENDIE E.,
LEGGE MICHAEL
Publication year - 2001
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.8755-8920.2001.450210.x
Subject(s) - oviduct , estrous cycle , platelet activating factor , endocrinology , ovary , medicine , biology , receptor , luteal phase , andrology , chemistry , hormone
PROBLEM: This study determined platelet activating factor (PAF) binding to ovaries and oviduct tissues to identify the location of PAF–tissue interaction.
METHOD: Mouse ovaries and oviducts taken during the estrous cycle and days 1, 4 and 7 post‐conception (pc) were analyzed by [H]PAF binding using frozen sections and autoradiography.
RESULTS: For the outer epithelium of estrous and metestrous ovaries there was no significant difference in PAF binding ( P >0.05); however, both stages were significantly different from proestrous ( P <0.001). Ovarian stroma PAF binding was significantly higher ( P <0.001) at estrous than metestrous, with no PAF binding at proestrous. Binding of PAF to estrous ovarian follicles was significantly greater ( P <0.001) than at proestrous and metestrous. Estrous oviduct binding of PAF was significantly increased for stroma, inner epithelium and lumen ( P <0.001 in all cases). At days 1 and 7 pc, all ovarian tissues had the greatest PAF binding with day 4 pc failing to bind PAF except for a significant decrease in corpora luteal binding ( P <0.001). Oviduct binding of PAF was greatest at day 1 pc.
CONCLUSION: Ovarian and oviduct PAF receptor expression corresponds to peak embryo PAF synthesis and establishes the basis for PAF–PAF receptor mediated early pregnancy signaling system.