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Painting Qa‐2 onto Ped slow Preimplantation Embryos Increases the Rate of Cleavage
Author(s) -
McELHINNY ABIGAIL S.,
EXLEY GINGER E.,
WARNER CAROL M.
Publication year - 2000
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.8755-8920.2000.440108.x
Subject(s) - embryo , cleavage (geology) , cell division , microbiology and biotechnology , chemistry , cell , biology , biochemistry , paleontology , fracture (geology)
PROBLEM: Qa‐2 protein, the Ped gene product, is linked to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Some GPI‐linked proteins can be spontaneously incorporated into the membranes of cells via a technique called “protein painting.” We investigated whether Qa‐2 could be painted onto T cells and embryos and whether the painted protein would be functional.
METHOD OF STUDY: Incorporation of Qa‐2 into the membranes of T cells and embryos was measured by FACScan and Immuno‐PCR, respectively. Function of Qa‐2 was measured by cell proliferation.
RESULTS: Qa‐2 was incorporated by T cells and embryos and was functional.
CONCLUSION: GPI‐linked Qa‐2 protein “painted” onto both T cells and preimplantation embryos is functional, as shown by increased proliferation of T cells after cross‐linking with anti‐Qa‐2 antibody, and increased rate of cleavage division of the embryos.