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Effects of Interferon‐Γ on Secretion of Vascular Endothelial Growth Factor by Endometrial Stromal Cells
Author(s) -
KAWANO YASUSHI,
MATSUI NAOHIKO,
KAMIHIGASHI SHOUKO,
NARAHARA HISASHI,
MIYAKAWA ISAO
Publication year - 2000
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.8755-8920.2000.430109.x
Subject(s) - stromal cell , vascular endothelial growth factor , endocrinology , angiogenesis , medicine , secretion , endometrium , biology , interferon gamma , cytokine , chemistry , vegf receptors
PROBLEM: The aim of this study was to clarify the physiological effects of interferon (IFN)‐Γ on secretion of vascular endothelial growth factor (VEGF) by endometrial stromal (ES) cells. METHOD OF STUDY: ES cells were obtained from human uterine endometrium by enzymic digestion and filtration. The effects of IFN‐Γ on production of VEGF by ES cells were examined by analyzing VEGF mRNA expression with Northern blotting analysis and by assaying VEGF protein. RESULTS: IFN‐Γ inhibited VEGF mRNA and protein expression by ES cells in a dose‐dependent manner. In ES cells treated with IFN‐Γ, VEGF production was not significant until 6 hr of incubation and was significantly affected after 6 hr of incubation, but decreased significantly after 12 to 48 hr. IFN‐Γ also suppressed VEGF mRNA expression by ES cells. CONCLUSIONS: ES cells produce VEGF, which may contribute to endometrial neovascularization and proliferation. IFN‐Γ may play an important role in regulating VEGF production by ES cells.